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Saliva crystallization method

 

G.M. Galati

 

R. Ganovic

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CLINICAL STUDY - R. GANOVIC                                                           Page 2 of 13


 

Crystallization of the saliva

            The ovulation related phenomenon of saliva crystallization in a fern-like pattern  was first observed in 1957 by Andreoli and Della Porta from University in Torino .  J.M.Biel Casals (Casals J.M.B., 1968) was the first  to investigate this phenomenon more extensively.   He examined 493 saliva samples and concluded that the intensity of crystallization is directly dependent on closeness of ovulation. The method used for determination of crystallization is simple: a drop of saliva is placed  on  a glass slide and left to dry at the room temperature. When completely dry, the sample is examined using  small magnification. 

 

Salivation  (Gayton C.A.,1981)

            Main salivary glands are: parotid, submandibular and sublingual glands;  there is also a lot of small buccal glands.  Daily salivation  ranges from 1000 to 1500 ml. Saliva consists of two kinds of secretion:  (1) serosus, containing ptyalin, enzyme (alpha-amylases) for starch digestion , and (2) mucous secretion which protects and  moists the oral cavity. The saliva pH is between 6.0 and 7.4 . Salivary glands are compound glands  consisting of  acinuses covered by secretory  glandular cells and system of small ducts conducting saliva into the  oral cavity.

            The secretion of saliva takes place in two stages; at the first  stage, acinuses  and, at the second,  ducts are involved.  The acinuses secrete the so called primary secretion  containing  saliva enzymes in ionized solution which, as regard ions, does not considerably differ from plasma. However, the ion composition of the primary secretion is significantly modified in the ducts  by two important active transportations.

            In the first place,  sodium ions are actively reabsorbed from  excretory ducts and  potassium ions are actively secreted  in exchange. Accordingly, the concentration of sodium  as well as chloride ions decreases while the concentration of potassium increases.  Secondly,  the bicarbonate ions are secreted into excretory ducts by way of enzyme carboanhydrasis  that is in epithelial  cells  of excretory ducts. In exchange for bicarbonates, additional  quantities of chloride ions are passively reabsorbed from excretory ducts . As a result of processes  of active transportation , in conditions of rest,  the concentration of sodium and chloride ions in saliva is about  15 mEq/lit each , being approximately 1/7 to 1/10 of their concentration of plasma. On the other hand, the concentration of potassium ions is about 30 mEq/lit being approximately 7 times higher than in plasma. The concentration of bicarbonate ions is about 50-90 mEq/lit , being 2 to 4 times higher than in plasma.

            When saliva is been secreted  more intensively, the concentration of ions in saliva considerably changes because the primary secrete in acinuses is secreted  20 times faster. That increase in fastness causes such an acceleration of the flow of the secrete  through  excretory ducts so that changes in its composition that occur during the passage  take place to a considerably lesser extent than they otherwise do.  Accordingly,  when saliva is secreted more intensively, the concentration of NaCl is higher than usual and amounts to 1/2 to 2/3 of  the one in plasma, while the concentration of potassium is lower than usual - only 4 times higher than in plasma.

            Due to the fact that a concentration of  potassium ions in saliva is high, any abnormal state accompanied by long-term hypersalivation can cause a serious loss of potassium ions and severe potassium defecit.

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